1.
Mem. Inst. Oswaldo Cruz
;
100(3): 269-272, May 2005. ilus
Article
in English
| LILACS
| ID: lil-411022
ABSTRACT
Fourteen hepatitis C virus (HCV) chronically infected patients were submitted to routine liver biopsy for histological evaluation. Liver samples were assayed to HCV-RNA by in situ hybridization, using digoxigenin labeled probe. HCV genotypes were found to be predominantly type 1 (71.4 percent), followed by genotype 3 (21.4 percent), and genotype 2 (7.2 percent). Alanine-aminotransferase levels were raised in 10 patients. The histopathological scores were minimal (21.4 percent), mild (57.2 percent), and moderate (21.4 percent). Viral RNA was detected in liver cells from nine patients (64.3 percent). ISH method provides localization and poor confirmation of HCV RNA in the liver tissue of HCV chronic patients.
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Hepacivirus/genetics , Hepatitis C, Chronic/virology , In Situ Hybridization/methods , Liver/virology , RNA, Viral/isolation & purification , Alanine Transaminase/blood , Biopsy , Digoxigenin , Formaldehyde , Genotype , Hepacivirus/isolation & purification , Hepatitis C, Chronic/pathology , Liver/pathology , Paraffin Embedding , Polymorphism, Restriction Fragment Length , Reverse Transcriptase Polymerase Chain Reaction , RNA, Viral/genetics , Severity of Illness Index
2.
Rev. bras. genét
;
6(2): 337-45, 1983.
Article
in English
| LILACS
| ID: lil-18290
Subject(s)
Child, Preschool , Child , Adult , Humans , Male , Female , Creatine Kinase , Menarche , Muscular Dystrophies , Pyruvate Kinase
3.
Rev. bras. genét
;
6(3): 527-38, 1983.
Article
in English
| LILACS
| ID: lil-18928